Review




Structured Review

Promega m-mlv rt 5x reaction buffer
M Mlv Rt 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+rt+5x+reaction+buffer/mgcl2/pm40659902-129-3-26
Average 90 stars, based on 1 article reviews
m-mlv rt 5x reaction buffer - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Incubation:

Article Title: KRAS-mutation status dependent effect of zoledronic acid in human non-small cell cancer preclinical models
Article Snippet: Three μg of total RNA were reverse transcribed from each sample using deoxy-NTPs (Promega, 0.5 mM each), a mixture of random primer and oligo dT (Promega, final concentration 3 μM), RNasin ® ribonuclease inhibitor (Promega, 20 U/reaction), M-MLV RT 5x Reaction Buffer (Promega, containing 250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl 2 and 50 mM DTT) and M-MLV Reverse Transcriptase RNase H Minus, Point Mutant (Promega, 200 U/reaction).

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C.

Article Title: Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole.
Article Snippet: Ten μL de M-MLV RT 5X Reaction Buffer, 2.5 μL dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dCTP y 10 mM dTTP) were added (Promega, USA), 1,2 μL Recombinant RNasin® Ribonuclease Inhibitor (40 U/μL), 2 μL of enzyme M-MLV RT (200 U/μL) y 10.3 μL of Nuclease free water was added, with a final volume of 50 μL.

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C. qPCR The total RNA was extracted from the SW480 cells and reverse transcribed to template cDNA.

Article Title: Rapid Detection of Tobacco Rattle Tobravirus in Viruliferous Paratrichodorus allius from Greenhouse and Field Specimens
Article Snippet: To each tube, 4 μl of M-MLV RT 5X Reaction Buffer (Promega, Madison, WI) 2.0 μl of a mixture containing 10 mM each of dATP, dCTP, dGTP, and dTTP (Invitrogen) and 0.2 μl (200u/μl) of M-MLV reverse transcriptase (Promega) were added.

Article Title: siRNA associated with immunonanoparticles directed against cd99 antigen improves gene expression inhibition in vivo in Ewing's sarcoma.
Article Snippet: 2013; 26: 318–329 Copyright © 2013 John Wiley & Sons, Ltd. wileyonlinelibrary.com/journal/jmr 321 40 U/ml, and 4 ml of M-MLV RT 5X reaction buffer (Promega, Fitchburg, WI, USA) for 1 h at 42 C. qPCR was performed using SYBR GreenER qPCR SuperMix (Invitrogen) using 5 ml of cDNA diluted to 1/20 (v/v) in 25 ml final volume.

Article Title: Gene Expression Profiling of Endometrial Adenocarcinomas Reveals Increased Apolipoprotein E Expression in Poorly Differentiated Tumors
Article Snippet: Introduction: Tumor grade is one of the most important prognostic factors in endometrioid endometrial adenocarcinoma.. Amplification of oncogenes, such as Her2/neu, or loss of function of tumor suppressor genes, such as p53, are known to be associated with poor prognosis, but additional factors influencing clinical behavior are likely to exist.. To examine the biological differences between low-grade and high-grade endometrioid endometrial adenocarcinomas, we compared gene expression in these 2 types of tumors.

Reverse Transcription:

Article Title: KRAS-mutation status dependent effect of zoledronic acid in human non-small cell cancer preclinical models
Article Snippet: Three μg of total RNA were reverse transcribed from each sample using deoxy-NTPs (Promega, 0.5 mM each), a mixture of random primer and oligo dT (Promega, final concentration 3 μM), RNasin ® ribonuclease inhibitor (Promega, 20 U/reaction), M-MLV RT 5x Reaction Buffer (Promega, containing 250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl 2 and 50 mM DTT) and M-MLV Reverse Transcriptase RNase H Minus, Point Mutant (Promega, 200 U/reaction).

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C.

Article Title: Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole.
Article Snippet: Ten μL de M-MLV RT 5X Reaction Buffer, 2.5 μL dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dCTP y 10 mM dTTP) were added (Promega, USA), 1,2 μL Recombinant RNasin® Ribonuclease Inhibitor (40 U/μL), 2 μL of enzyme M-MLV RT (200 U/μL) y 10.3 μL of Nuclease free water was added, with a final volume of 50 μL.

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C. qPCR The total RNA was extracted from the SW480 cells and reverse transcribed to template cDNA.

Article Title: Rapid Detection of Tobacco Rattle Tobravirus in Viruliferous Paratrichodorus allius from Greenhouse and Field Specimens
Article Snippet: To each tube, 4 μl of M-MLV RT 5X Reaction Buffer (Promega, Madison, WI) 2.0 μl of a mixture containing 10 mM each of dATP, dCTP, dGTP, and dTTP (Invitrogen) and 0.2 μl (200u/μl) of M-MLV reverse transcriptase (Promega) were added.

Article Title: siRNA associated with immunonanoparticles directed against cd99 antigen improves gene expression inhibition in vivo in Ewing's sarcoma.
Article Snippet: 2013; 26: 318–329 Copyright © 2013 John Wiley & Sons, Ltd. wileyonlinelibrary.com/journal/jmr 321 40 U/ml, and 4 ml of M-MLV RT 5X reaction buffer (Promega, Fitchburg, WI, USA) for 1 h at 42 C. qPCR was performed using SYBR GreenER qPCR SuperMix (Invitrogen) using 5 ml of cDNA diluted to 1/20 (v/v) in 25 ml final volume.

Article Title: Gene Expression Profiling of Endometrial Adenocarcinomas Reveals Increased Apolipoprotein E Expression in Poorly Differentiated Tumors
Article Snippet: Introduction: Tumor grade is one of the most important prognostic factors in endometrioid endometrial adenocarcinoma.. Amplification of oncogenes, such as Her2/neu, or loss of function of tumor suppressor genes, such as p53, are known to be associated with poor prognosis, but additional factors influencing clinical behavior are likely to exist.. To examine the biological differences between low-grade and high-grade endometrioid endometrial adenocarcinomas, we compared gene expression in these 2 types of tumors.

Real-time Polymerase Chain Reaction:

Article Title: KRAS-mutation status dependent effect of zoledronic acid in human non-small cell cancer preclinical models
Article Snippet: Three μg of total RNA were reverse transcribed from each sample using deoxy-NTPs (Promega, 0.5 mM each), a mixture of random primer and oligo dT (Promega, final concentration 3 μM), RNasin ® ribonuclease inhibitor (Promega, 20 U/reaction), M-MLV RT 5x Reaction Buffer (Promega, containing 250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl 2 and 50 mM DTT) and M-MLV Reverse Transcriptase RNase H Minus, Point Mutant (Promega, 200 U/reaction).

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C.

Article Title: Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole.
Article Snippet: Ten μL de M-MLV RT 5X Reaction Buffer, 2.5 μL dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dCTP y 10 mM dTTP) were added (Promega, USA), 1,2 μL Recombinant RNasin® Ribonuclease Inhibitor (40 U/μL), 2 μL of enzyme M-MLV RT (200 U/μL) y 10.3 μL of Nuclease free water was added, with a final volume of 50 μL.

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C. qPCR The total RNA was extracted from the SW480 cells and reverse transcribed to template cDNA.

Article Title: Rapid Detection of Tobacco Rattle Tobravirus in Viruliferous Paratrichodorus allius from Greenhouse and Field Specimens
Article Snippet: To each tube, 4 μl of M-MLV RT 5X Reaction Buffer (Promega, Madison, WI) 2.0 μl of a mixture containing 10 mM each of dATP, dCTP, dGTP, and dTTP (Invitrogen) and 0.2 μl (200u/μl) of M-MLV reverse transcriptase (Promega) were added.

Article Title: siRNA associated with immunonanoparticles directed against cd99 antigen improves gene expression inhibition in vivo in Ewing's sarcoma.
Article Snippet: 2013; 26: 318–329 Copyright © 2013 John Wiley & Sons, Ltd. wileyonlinelibrary.com/journal/jmr 321 40 U/ml, and 4 ml of M-MLV RT 5X reaction buffer (Promega, Fitchburg, WI, USA) for 1 h at 42 C. qPCR was performed using SYBR GreenER qPCR SuperMix (Invitrogen) using 5 ml of cDNA diluted to 1/20 (v/v) in 25 ml final volume.

Article Title: Gene Expression Profiling of Endometrial Adenocarcinomas Reveals Increased Apolipoprotein E Expression in Poorly Differentiated Tumors
Article Snippet: Introduction: Tumor grade is one of the most important prognostic factors in endometrioid endometrial adenocarcinoma.. Amplification of oncogenes, such as Her2/neu, or loss of function of tumor suppressor genes, such as p53, are known to be associated with poor prognosis, but additional factors influencing clinical behavior are likely to exist.. To examine the biological differences between low-grade and high-grade endometrioid endometrial adenocarcinomas, we compared gene expression in these 2 types of tumors.

Concentration Assay:

Article Title: KRAS-mutation status dependent effect of zoledronic acid in human non-small cell cancer preclinical models
Article Snippet: Three μg of total RNA were reverse transcribed from each sample using deoxy-NTPs (Promega, 0.5 mM each), a mixture of random primer and oligo dT (Promega, final concentration 3 μM), RNasin ® ribonuclease inhibitor (Promega, 20 U/reaction), M-MLV RT 5x Reaction Buffer (Promega, containing 250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl 2 and 50 mM DTT) and M-MLV Reverse Transcriptase RNase H Minus, Point Mutant (Promega, 200 U/reaction).

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C.

Article Title: Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole.
Article Snippet: Ten μL de M-MLV RT 5X Reaction Buffer, 2.5 μL dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dCTP y 10 mM dTTP) were added (Promega, USA), 1,2 μL Recombinant RNasin® Ribonuclease Inhibitor (40 U/μL), 2 μL of enzyme M-MLV RT (200 U/μL) y 10.3 μL of Nuclease free water was added, with a final volume of 50 μL.

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C. qPCR The total RNA was extracted from the SW480 cells and reverse transcribed to template cDNA.

Article Title: Rapid Detection of Tobacco Rattle Tobravirus in Viruliferous Paratrichodorus allius from Greenhouse and Field Specimens
Article Snippet: To each tube, 4 μl of M-MLV RT 5X Reaction Buffer (Promega, Madison, WI) 2.0 μl of a mixture containing 10 mM each of dATP, dCTP, dGTP, and dTTP (Invitrogen) and 0.2 μl (200u/μl) of M-MLV reverse transcriptase (Promega) were added.

Article Title: siRNA associated with immunonanoparticles directed against cd99 antigen improves gene expression inhibition in vivo in Ewing's sarcoma.
Article Snippet: 2013; 26: 318–329 Copyright © 2013 John Wiley & Sons, Ltd. wileyonlinelibrary.com/journal/jmr 321 40 U/ml, and 4 ml of M-MLV RT 5X reaction buffer (Promega, Fitchburg, WI, USA) for 1 h at 42 C. qPCR was performed using SYBR GreenER qPCR SuperMix (Invitrogen) using 5 ml of cDNA diluted to 1/20 (v/v) in 25 ml final volume.

Article Title: Gene Expression Profiling of Endometrial Adenocarcinomas Reveals Increased Apolipoprotein E Expression in Poorly Differentiated Tumors
Article Snippet: Introduction: Tumor grade is one of the most important prognostic factors in endometrioid endometrial adenocarcinoma.. Amplification of oncogenes, such as Her2/neu, or loss of function of tumor suppressor genes, such as p53, are known to be associated with poor prognosis, but additional factors influencing clinical behavior are likely to exist.. To examine the biological differences between low-grade and high-grade endometrioid endometrial adenocarcinomas, we compared gene expression in these 2 types of tumors.

Mutagenesis:

Article Title: KRAS-mutation status dependent effect of zoledronic acid in human non-small cell cancer preclinical models
Article Snippet: Three μg of total RNA were reverse transcribed from each sample using deoxy-NTPs (Promega, 0.5 mM each), a mixture of random primer and oligo dT (Promega, final concentration 3 μM), RNasin ® ribonuclease inhibitor (Promega, 20 U/reaction), M-MLV RT 5x Reaction Buffer (Promega, containing 250 mM Tris-HCl, pH 8.3, 375 mM KCl and 15 mM MgCl 2 and 50 mM DTT) and M-MLV Reverse Transcriptase RNase H Minus, Point Mutant (Promega, 200 U/reaction).

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C.

Article Title: Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole.
Article Snippet: Ten μL de M-MLV RT 5X Reaction Buffer, 2.5 μL dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dCTP y 10 mM dTTP) were added (Promega, USA), 1,2 μL Recombinant RNasin® Ribonuclease Inhibitor (40 U/μL), 2 μL of enzyme M-MLV RT (200 U/μL) y 10.3 μL of Nuclease free water was added, with a final volume of 50 μL.

Article Title: Effects of thalidomide on growth and VEGF-A expression in SW480 colon cancer cells
Article Snippet: Following incubation at 70°C for 10 min, the mixture was immediately placed in ice bath for 2 min. M-MLV RT 5X reaction buffer, M-MLV Reverse Transcriptase RNase Minus, and 10 mM dNTP (Promega Corporation) were added to the mixture and then incubated at 42°C for 1 h, followed by 70°C for 10 min. cDNA was stored at −80°C. qPCR The total RNA was extracted from the SW480 cells and reverse transcribed to template cDNA.

Article Title: Rapid Detection of Tobacco Rattle Tobravirus in Viruliferous Paratrichodorus allius from Greenhouse and Field Specimens
Article Snippet: To each tube, 4 μl of M-MLV RT 5X Reaction Buffer (Promega, Madison, WI) 2.0 μl of a mixture containing 10 mM each of dATP, dCTP, dGTP, and dTTP (Invitrogen) and 0.2 μl (200u/μl) of M-MLV reverse transcriptase (Promega) were added.

Article Title: siRNA associated with immunonanoparticles directed against cd99 antigen improves gene expression inhibition in vivo in Ewing's sarcoma.
Article Snippet: 2013; 26: 318–329 Copyright © 2013 John Wiley & Sons, Ltd. wileyonlinelibrary.com/journal/jmr 321 40 U/ml, and 4 ml of M-MLV RT 5X reaction buffer (Promega, Fitchburg, WI, USA) for 1 h at 42 C. qPCR was performed using SYBR GreenER qPCR SuperMix (Invitrogen) using 5 ml of cDNA diluted to 1/20 (v/v) in 25 ml final volume.

Article Title: Gene Expression Profiling of Endometrial Adenocarcinomas Reveals Increased Apolipoprotein E Expression in Poorly Differentiated Tumors
Article Snippet: Introduction: Tumor grade is one of the most important prognostic factors in endometrioid endometrial adenocarcinoma.. Amplification of oncogenes, such as Her2/neu, or loss of function of tumor suppressor genes, such as p53, are known to be associated with poor prognosis, but additional factors influencing clinical behavior are likely to exist.. To examine the biological differences between low-grade and high-grade endometrioid endometrial adenocarcinomas, we compared gene expression in these 2 types of tumors.



Similar Products

90
Promega m-mlv rt 5x reaction buffer
M Mlv Rt 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+rt+5x+reaction+buffer/mgcl2/pm40659902-129-3-26
Average 90 stars, based on 1 article reviews
m-mlv rt 5x reaction buffer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega m-mlv rt 5x reaction buffer cat.m1705
M Mlv Rt 5x Reaction Buffer Cat.M1705, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+rt+5x+reaction+buffer/m+mlv+reverse+transcriptase/pm40247073-294-46-52
Average 90 stars, based on 1 article reviews
m-mlv rt 5x reaction buffer cat.m1705 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega 5x m-mlv rt reaction buffer
5x M Mlv Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+rt+5x+reaction+buffer/mgcl2/pmc11545096-147-14-21
Average 90 stars, based on 1 article reviews
5x m-mlv rt reaction buffer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega m. mlv rt 5x reaction buffer
M. Mlv Rt 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+rt+5x+reaction+buffer/mgcl2/pm35636533-104-14-20
Average 90 stars, based on 1 article reviews
m. mlv rt 5x reaction buffer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega rt buffer m-mlv rt 5x reaction buffer
Rt Buffer M Mlv Rt 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+rt+5x+reaction+buffer/mgcl2/10__1111_slash_epp__12676-381-0-7
Average 90 stars, based on 1 article reviews
rt buffer m-mlv rt 5x reaction buffer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega 5x m-mlv rt 5x reaction buffer
5x M Mlv Rt 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+rt+5x+reaction+buffer/mgcl2/pm26498029-60-38-44
Average 90 stars, based on 1 article reviews
5x m-mlv rt 5x reaction buffer - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results